Platelet-rich fibrin (PRF) is an autologous biomaterial extensively used in regenerative dentistry because of its sustained release of growth factors. Among its various forms, standard PRF (S-PRF) and advanced PRF (A-PRF) differ in their centrifugation protocols, which may affect their biological properties and regenerative potential. This study aimed to compare the release of growth factors from sticky bone prepared using S-PRF and A-PRF. This in vitro experimental study was conducted using blood samples collected from healthy volunteers (n = 10). Sticky bone was prepared by mixing demineralized freeze-dried bone allograft (DFDBA) with either S-PRF or A-PRF. Growth factor analysis—including Angiopoietin-2, EGF, EPO, FGF, G-CSF, GM-CSF, PDGF-AA, PDGF-BB, SCF, TGF-α, VEGF, and HGF—was performed using the LEGENDplex multi-analyte flow cytometry assay. The A-PRF group demonstrated significantly higher levels of EPO, Angiopoietin-2, G-CSF, and HGF compared with the S-PRF group (p < 0.05), whereas FGF levels were similar between the two groups. Within the limitations of this study, A-PRF appears to be a more effective option for sticky bone preparation and may enhance bone regeneration outcomes.
