Intracanal medication reduces micro-organisms after cleaning & shaping a root canal. Long-term exposure to some intra-canal medicament decreases fracture resistance of radicular dentin due to its strong alkalinity in long-term exposure. We studied the resistance of human root dentin to fracture following the application of intracanal medicament on the 7th and 10th days. Thirty-six human permanent extracted teeth with singleroot were collected. They were cleaned and shaped till Hand ProTaper F2. After de-coronation, roots were divided randomly as Group A - Calcium Hydroxide, Group B - Propolis, and Group C - Chitosan Mixed with Calcium Hydroxide, having 12 teeth respectively. The respective intracanal medicament paste was introduced in root canals. We divided samples into two subgroups. There were 6 teeth in each group. We checked the fracture resistance on the 7th and 10th days by using the universal testing machine. The fracture resistance was recorded in MPa automatically in the universal testing machine. We used one-way ANOVA followed by unpaired and paired for individual group comparisons. A p-value of Results- Mean values of fracture load (N) for 7days between groups Ca(OH)2, Propolis, and Chitosan + Ca(OH)2 was not significant Also, mean values of fracture load (N) at 10days when compared individually in group Ca(OH)2, Propolis and Chitosan + Ca(OH)2 there was no significant difference. Intracanal medicaments used decreased the resistance of radicular dentin to fracture. Propolis showed the least fracture resistance followed by Chitosan mixed with calcium hydroxide and Calcium Hydroxide alone.