Serratiopeptidase, a proteolytic enzyme with anti-inflammatory and fibrinolytic properties, has shown potential in wound healing, though its specific effects on oral mucosal repair remain underexplored. This study employed an integrated approach combining histopathological analysis with biochemical and hematological assessment in a rabbit oral mucosal wound model. Fifteen rabbits were allocated into control and serratiopeptidase-treated groups, with samples collected on days 3 and 10 post-intervention. Histological evaluation, complete blood count analysis, and serum inflammatory markers (CRP, LDH) were performed. Histopathological examination revealed significantly accelerated healing in serratiopeptidase-treated animals. At day 3, treated lesions showed advanced granulation tissue formation, extensive angiogenesis, and early re-epithelialization, whereas controls showed persistent inflammation. By day 10, treated animals exhibited complete re-epithelialization with well-organized tissue architecture, while controls displayed incomplete healing. These findings correlated with hematological data showing significantly reduced neutrophil counts (1.66±0.59 versus 2.70±1.73×10⁹/L, p=0.009) and increased lymphocyte counts (3.01±1.33 versus 1.78±1.06×10⁹/L, p=0.006) in the treated versus control groups at day 10. Inflammatory biomarkers demonstrated a biphasic response with early elevation followed by rapid normalization in treated animals. Serratiopeptidase significantly enhances oral mucosal healing by coordinating modulation of the inflammatory response, promoting angiogenesis, and accelerating re-epithelialization. The enzyme demonstrates pro-resolution properties that optimize the entire healing cascade.
