This study investigates the effects of Aloe Vera, injectable platelet-rich fibrin (i-PRF), and their combination on the proliferation of mesenchymal stem cells (MSCs) derived from human hair follicles. MSCs were isolated using enzymatic digestion and characterized by flow cytometry for surface markers indicative of mesenchymal origin. The MTT assay was employed to assess cell proliferation and cytotoxicity at varying concentrations (0.01% to 1%) of Aloe Vera, i-PRF, and their combination. Aloe Vera alone showed limited proliferative effect at lower concentrations and significant cytotoxicity at 1% (p = 0.0002). In contrast, i-PRF significantly enhanced MSC proliferation at 0.1% (p = 0.0028) and 1% (p = 0.0117). When used in combination, Aloe Vera and i-PRF demonstrated a synergistic effect, with significantly increased MSC proliferation at 0.85% (p = 0.00135) and 1% (p = 0.00243). These findings challenge the prevailing notion that 100% purity or higher concentrations of Aloe Vera are inherently beneficial, emphasizing the need for optimal dosing. The study underscores the regenerative potential of combining plant-based bioactives with autologous biological agents and supports further exploration into their application for hair follicle regeneration and the treatment of androgenic alopecia.
