Biopolymer-based delivery systems incorporating plant-derived phytoconstituents are increasingly explored for improving human health and wellbeing through multifunctional biomedical applications. In oral diseases, oxidative stress and inflammation play key roles in disease progression. Gymnema sylvestre and Curcuma longa exhibit established antioxidant and anti-inflammatory properties; however, their combined incorporation into an alginate-based biocomposite and systematic in vitro evaluation relevant to oral disease management remain limited. To develop an alginate-based biocomposite incorporating Gymnema sylvestre and Curcuma longa extracts and to evaluate its cytotoxic, antioxidant, anti-inflammatory, and anticoagulant activities through in vitro assays relevant to oral disease management. Aqueous extracts of Gymnema sylvestre leaves and Curcuma longa rhizomes were incorporated into a sodium alginate matrix to formulate a stable biocomposite. Cytotoxicity was assessed using the brine shrimp lethality assay to determine preliminary safety for human health applications. Antioxidant activity was evaluated by DPPH radical scavenging, anti-inflammatory activity by protein denaturation inhibition, and anticoagulant activity by clotting time measurement. Experiments were conducted in triplicate and analyzed using one-way ANOVA and independent sample t-tests. The biocomposite demonstrated concentration-dependent biological activity with mild cytotoxicity. Antioxidant and anti-inflammatory activities increased progressively, while anticoagulant assessment showed a modest prolongation of clotting time. Overall, the biocomposite exhibited efficacy comparable to standard reference drugs (p > 0.05). The alginate-based phytochemical biocomposite demonstrated favorable in vitro biological activity and safety, highlighting its potential relevance in oral disease management and promotion of oral health and wellbeing.
