Dental impressions serve as a source of infection transmission from dental clinics to dental labs. To evaluate the knowledge, attitude, and practice of disinfection methods for dental impressions among dental specialists, post-graduates, and dental technicians through an online survey. A descriptive and analytical online survey on the knowledge, attitudes, and practices of disinfection of impressions was conducted among 112 dental specialists, post-graduates, and dental technicians in the southern part of India from January 2024 to March 2024. It consists of 20 questions, and the participants include 23 post-graduates, 40 academicians and private practitioners, 31 private practitioners, 13 academicians, and 5 technicians. The majority of the participants were aware of the types of disease transmission through impression. Still, they thought that washing the impression under running tap water removes all types of infection, which is wrong. Only 60% of the infection is removed through this method. Additionally, the majority of participants are unaware of the composition of the disinfectant solution. Most participants are unaware of the level of disinfection of disinfectants and the specific disinfectants suitable for various impression materials. Hence, vigorous awareness and continuing education programs should be insisted upon among dentists to prevent cross-contamination in dental offices and laboratories.