We can avoid many complications before endodontic treatment by knowing the variation between maxillary right and left premolars and molars regarding canals, roots length, and roots numbers. The data collected and were compared between right and left maxillary premolar and molar teeth by using CBCT. All the maxillary first and second molars had three roots. The average root length of maxillary second right molar (meso buccal) was 13.19 mm, (Disto buccal) was 12.22 mm, and (palatal) was 13.13 mm. The average root length of maxillary second left molar MB was 13.12 mm, DB was 12.17 mm, and P was 13.90 mm. The average root length of the maxillary first right molar MB was 13.27 mm, DB was 12.37 mm, and P was 13.98 mm. The average root length of maxillary first left molar MB was 13.28 mm, DB was 12.43 mm, and P was 14.03 mm. There is no significant difference in length roots, root numbers, and canals number between the maxillary right and left premolars and molars.