The Cementoenamel Junction is considered an important landmark for locating the pulp chambers and canals orifices as Krasaner and Rankow found. The main objective of this study is to assess the knowledge and awareness level of root canal orifice laws and detection methods among dentists, interns, and dental students in Saudi Arabia. An observational Cross-sectional study by online questionnaire survey, the questions are formulated by the authors in Saudi Arabia. The sample size was estimated using the Qualtrics calculator with an a-1 confidence level of 95%; a sample size of 384. The data were tabulated on Microsoft Excel and analyzed using IBM Statistical Package for the Social Sciences (SPSS) version 22.0. As regard to current professional status highest percent of participants who had a high knowledge score (29.1%) were dentists. More than half of those with high knowledge scores were studying dentistry in Saudi Arabia. Participants in the 4th year had the highest percent of low knowledge scores. These differences are statistically significant with P-value < .05. The study shows that the knowledge of dentists regarding root canal orifices laws and detection methods was not satisfactory only 62.1% out of 419 had high knowledge, 33.4% had medium knowledge, and 4.5% had low knowledge. Therefore, participants require an understanding of the concepts behind CBCT and related technologies and other methods of detection, making appropriate training essential for every member of the dental team.
