Matching tooth color with restoration is one of the most difficult tasks in restorative dentistry. One of the important aspects of dental education is teaching and learning color matching. The purpose of this study was to investigate the effect of the teaching method on improving the ability to determine color by dental students. In this study, dental students were randomly divided into two groups. The first group was taught the methods of tooth color matching in person and practically. In the second group, a training booklet was used for training. The students of each group were asked before, after, and after 1 month to determine the color of the samples using the Vita Classic color determination series. The evaluation of student's ability to determine color was done with the Pearson Chi-Square test with a sample size of 18 or 20 and with a confidence level of 95%. The training group with the booklet was significantly more successful in determining the color of the fourth sample (P< 0.01). In none of the studied groups and any of the color samples used, the results one month after the training were not better than the results before the training started. Also, there was no significant relationship between the accuracy of color determination with the gender of the students and the use of glasses. Therefore, the use of glasses and gender did not affect the accuracy of color determination. According to the obtained results, it can be concluded that training with a booklet is more effective than practical training for dental students in improving the accuracy of color determination.