External apical root resorption results in blunting and shortening of roots with open apices that present a major challenge in obtaining a complete three-dimensional seal of the root canal system. In recent years the treatment option in the management of a case with external root resorption is the use of an apical barrier. This case report describes the novel use of amnion membrane matrix and Bio dentine as an apical barrier in the management of an apical root resorption case. Access opening was initiated followed by thorough cleaning and shaping of the root canals. Calcium hydroxide was placed for 2 weeks within the root canal as an intracanal medicament. In a subsequent appointment, an amnion membrane was placed at the root apex to serve as an internal matrix followed by a 5 mm apical plug with Biodentine. This was followed by post space preparation and placement of glass fiber post in the canal along with composite core build-up. Teeth were restored with full veneer porcelain fused to metal crowns. After eighteen months, there were no clinical signs or symptoms, and the formation of a calcific tissue apical to the Biodentine barrier. The favorable results, in this case, suggest that amnion membrane matrix and Biodentine plugs can be used to treat apical root resorption predictably.