Several methods of using teeth to estimate age at death are based on macroscopic, microscopic, and biochemical analyses of teeth. In addition to being very complex and expensive, microscopic and biochemical, methods destroy dental tissue and therefore make it impossible to use dental findings for further studies. The purpose of this article is to review the non-destructive methods of estimating the age of remains through teeth for immature people by examining the evolution of the dental system and for adults by examining the physiological analysis of dental tissues. Due to the regular nature of teeth formation and growth, "dental evolution" is the most widely used method to estimate the age of the remains of immature people. The traces of aging can be examined in the three different processes of the development of the dental system, "calcification", "teeth growth" and "complete closure of the end of the root" in pre-prepared tables and charts. Its wear begins with the eruption of permanent teeth. The prevalence of wear in a population is a function of age and can be used in age estimation. The continued formation of secondary dentin is also a biological response to aging. With age, the pulp area gradually decreases due to the continued deposition of secondary dentin. Measuring the trend of this decline using radiological photographs is used as an indicator in age estimation. The translucent end of teeth roots is also related to aging in adults and its length can be measured with proper accuracy. Although in archaeological samples, it still needs more investigation.
