TY  - JOUR
T1  - Comparison of Cytotoxicity Between Novel Obturating Material and Zinc Oxide Eugenol in Periapical Stem Cells
A1  - Manisha Bala Rathy Rajadurai
A1  - Lavanya Govindaraju
JF  - Annals of Dental Specialty
JO  - Ann Dent Spec
SN  - 2347-2022
Y1  - 2025
VL  - 13
IS  - 1
DO  - 10.51847/3TNOcDFFPA
SP  - 12
EP  - 18
N2  - Pulpectomy is an essential dental procedure aimed at preserving primary teeth affected by severe infections or irreversible pulpitis. Zinc oxide eugenol (ZOE), a commonly used obturating material, presents limitations including slow resorption, cytotoxicity, and potential adverse effects on developing permanent teeth. In response to these challenges, a novel obturating material combining zinc oxide, calcium hydroxide, and metronidazole has been developed. This study aimed to compare the cytotoxicity of the novel obturating material with that of ZOE using an MTT assay. Cytotoxicity was assessed using mouse fibroblast cells (3T3-L1) cultured in DMEM-F12 medium. The cells were treated with various concentrations of the novel material and ZOE, followed by MTT assay to measure cell viability. Absorbance was recorded at 570 nm, and statistical analysis was conducted using SPSS software. At lower concentrations (2.5 to 5 µl/ml), both materials exhibited minimal cytotoxicity, with cell viability exceeding 90%. However, as the concentration increased, both materials demonstrated a dose-dependent decline in cell viability. At 60 µl/ml, the novel material exhibited 56.63% cell viability, whereas ZOE showed 45.36%, indicating greater cytotoxicity associated with ZOE at higher concentrations. Intra-group analysis revealed significant cytotoxic differences between low and high concentrations for both materials. The novel obturating material demonstrates lower cytotoxicity than ZOE, particularly at lower concentrations, positioning it as a safer and more biocompatible alternative for pulpectomy in pediatric dentistry. Further clinical trials are warranted to validate these findings and evaluate long-term outcomes.
UR  - https://annalsofdentalspecialty.net.in/article/comparison-of-cytotoxicity-between-novel-obturating-material-and-zinc-oxide-eugenol-in-periapical-st-y92hjfuaxupqmux
ER  -