%0 Journal Article
%T Comparative Effect of Contamination and Decontamination of Two Adhesive Systems by Blood and Haemostatic Agent on The Bond Strength to Dentin: In Vitro
%A Aseel Fareed Faisal
%A Shibu Thomas Mathew
%J Annals of Dental Specialty
%@ 2347-2022
%D 2020
%V 8
%N 1
%P 34-43
%X Aim: This study aimed to detect how blood and haemostatic agent contamination affects the shear bond strength of self-etch and total-etch adhesives to dentin and to compare the difference in bond strength due to contamination and decontamination after adhesive light curing.

Materials and Methods: 130 sound wet human molars were used to prepare a flat dentin surface on its buccal side. Randomly, they were divided into 2 groups (n=65) based on the adhesives Group 1: (Prime & Bond Select™ Dentsply Detrey GmbH, 78467 Konstanz, Germany) and Group 2: (Prime & Bond Etch & Rinse™ Dentsply Detrey GmbH, 78467 Konstanz, Germany). Each main group has further divided into 3 sub-groups (n-30) based on the type of contaminants used (blood and haemostatic agent). Based on the decontaminants used (air, water, NaOCl) after adhesive light curing, these contaminated groups were further subdivided into 3 sub-groups (n=10). Blood and haemostatic were applied after the application of the adhesives. Nanoceramic composite resin was applied as a cylindrical disc on top of dentine surface, that were subjected to shear bond strength test (Istrone®). A stereomicroscopic evaluation was done to evaluate the modes of failure.

Statistical analysis: Three-way ANOVA following Scheffe post hoc test using software SPSS version 20 was used.

Results: Self-etch adhesive system revealed the highest shear bond strength than a total-etch adhesive system after contamination with blood or haemostatic agent. The mean difference of shear bond strength (MPa) in both total-etch and self-etch between blood and the hemostatic agent was statistically significant (p
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