TY  - JOUR
T1  - Chromosomal and Plasmid-Associated Gene Profiles of Enterococcus Faecalis: Adaptive Patterns Across Endodontic Environments
A1  - Alexandra Castillo-Guevara
A1  - Silvia Barrientos-Sánchez
A1  - Mayra A. Tellez-Corral
A1  - Adriana Rodríguez-Ciodaro
A1  - Catalina Méndez-De la Espriella
A1  - Nidia E. De Leon-Frias
A1  - Oscar E. Gómez-Cárdena
A1  - Hugo Díez-Ortega
JF  - Annals of Dental Specialty
JO  - Ann Dent Spec
SN  - 2347-2022
Y1  - 2025
VL  - 13
IS  - 4
DO  - 10.51847/dNcupIjFt3
SP  - 23
EP  - 29
N2  - Enterococcus faecalis is a microorganism frequently implicated in endodontic infections, capable of colonising both biological tissues and inert surfaces. However, the relationship between its chromosomal and plasmid gene repertoires and the type of endodontic environment it inhabits remains poorly elucidated. To analyse the distribution of E. faecalis virulence genes and their association with the ecological origin of biotic and abiotic isolates. Seventy-five E. faecalis isolates obtained from biotic and abiotic samples collected between 2018 and 2022 were analysed. Taxonomic identification was performed using a MALDI-TOF MS system, and virulence genes were detected by conventional PCR. Gene profiles and their association with the ecological origin of the isolates were evaluated using Factor Analysis of Mixed Data (FAMD), followed by Ward’s hierarchical clustering and K-means optimisation. Three genetic clusters of E. faecalis were identified and differentiated by their gene repertoires: chromosomal (ace, efaA, gelE), plasmid (asa, asa373, cylA), and mixed (esp). Chromosomal repertoires predominated in biotic isolates, whereas plasmid repertoires were more frequent in abiotic isolates, indicating a significant genetic structure according to ecological origin (p < 0.05). The gene repertoires of E. faecalis tend to cluster according to their chromosomal or plasmid nature, reflecting distinct patterns of ecological adaptation between biotic and abiotic endodontic environments.
UR  - https://annalsofdentalspecialty.net.in/article/chromosomal-and-plasmid-associated-gene-profiles-of-enterococcus-faecalis-adaptive-patterns-across-kq6cuqpcihkpn4f
ER  -